Nano Safety and Bioactivity

The Nano Safety and Bioactivity (NSB) Research Team is at the forefront of research and development in predictive models and testing methods tailored to environmental and health safety evaluations, bioactivity assessments, and efficacy studies. Through our innovative approaches and cutting-edge technology, we support both research institutes and industries across Thailand.

Our expertise spans a range of model systems, including in vitro cell culture with 2D and 3D models that mimic digestive, vascular, skin, and respiratory organs; ex vivo human skin models; and in vivo zebrafish models. These tools enable us to provide comprehensive testing solutions for a wide array of applications.

We provide testing services and work closely with research institutes, companies, and regulatory bodies, playing a significant role in supporting the development of regulatory frameworks and industry standards in Thailand. Our work supports sectors such as nanotechnology, cosmeceuticals, nutraceuticals, food and feed industries, and health and medicine, helping to advance safer, more effective products and innovations in these fields.

Research Scope

1. Development of Models and Testing Methods

The research team focuses on developing diverse study models, including in vitro, in vivo, and ex vivo, to support academic research and studies relevant to various industries. Additionally, comprehensive testing methods are developed to evaluate the safety and efficacy of substances or products, ensuring high standards for both research and testing services.

2. Services and Custom Research Projects

The team provides testing services and contract research projects, utilizing models developed in-house alongside testing methods that comply with OECD Test Guidelines and proprietary methods created by the team. These offerings are tailored to meet the specific needs of clients and research collaborators.

3. Development of Nanoparticles

Research and development focus on organic nanoparticles and inorganic nanoparticles for applications in various fields such as medicine, cosmetics, and environmental studies. The goal is to produce innovative products with unique properties and high potential for diverse uses.

4. Development of Cultured Meat Production Platforms

The team conducts research and development of cultured meat by integrating 3D printing technology with stem cell sources from economically significant animals. This initiative aims to create a sustainable production process that aligns with the growing demand for alternative protein sources.

5. Development of Zebrafish Testing Laboratories to Meet International Standards

Efforts are focused on enhancing zebrafish testing laboratory to achieve OECD GLP (Good Laboratory Practice) certification. The objective is to elevate research standards and enhance the competitiveness of Thai industries at the international level.

Selected publications

• Chanajon P, Hamzeh A, Tian F, Roytrakul S, Oluwagunwa OA, Kadam D, Aluko RE, Aueviriyavit S, Wongwanakul R, Yongsawatdigul J. Hypotensive effect of potent angiotensin-I-converting enzyme inhibitory peptides from corn gluten meal hydrolysate: Gastrointestinal digestion and transepithelial transportation modifications. Food Chem. 2025 Jan 1;462:140953. https://doi.org/10.1016/j.foodchem.2024.140953
• Klinngam W, Rungkamoltip P, Wongwanakul R, Joothamongkhon J, Du-A-Man S, Khongkow M, Asawapirom U, Iempridee T, Ruktanonchai U. Skin Rejuvenation Efficacy and Safety Evaluation of Kaempferia parviflora Standardized Extract (BG100) in Human 3D Skin Models and Clinical Trial. Biomolecules. 2024 Jun 29;14(7):776. https://doi.org/10.3390/biom14070776
• Dechbumroong P, Hu R, Keaswejjareansuk W, Namdee K, Liang XJ. Recent advanced lipid-based nanomedicines for overcoming cancer resistance. Cancer Drug Resist. 2024 Jun 21;7:24. https://doi.org/10.20517/cdr.2024
• Lapmanee S, Bhubhanil S, Wongchitrat P, Charoenphon N, Inchan A, Ngernsutivorakul T, Dechbumroong P, Khongkow M, Namdee K. Assessing the Safety and Therapeutic Efficacy of Cannabidiol Lipid Nanoparticles in Alleviating Metabolic and Memory Impairments and Hippocampal Histopathological Changes in Diabetic Parkinson’s Rats. Pharmaceutics. 2024 Apr 8;16(4):514. https://doi.org/10.3390/pharmaceutics16040514
• Malila Y, Charoensuk D, Srimarut Y, Saensa-Ard S, Petpiroon N, Kunyanee C, Rattanawongsa W, Saenmuangchin R, Klamchuen A, Kangwansupamonkon W, Aueviriyavit S. Muscle Proteins, Technological Properties, and Free Amino Acids of Epaxial Muscle Collected from Asian Seabass (Lates calcarifer) at Different Postmortem Durations. Animals (Basel). 2024 Oct 1;14(19):2837. https://doi.org/10.3390/ani14192837
• Rittidach T, Sillapaprayoon S, Chantho V, Pimtong W, Charoenphandhu N, Thongbunchoo J, Krishnamra N, Bootchanont A, Porjai P, Pon-On W. Investigation on the physical properties and biocompatibility of zirconia-alumina-silicate@diopside composite materials and its in vivo toxicity study in embryonic zebrafish. RSC Adv. 2023 Oct 18;13(44):30575-30585. https://doi.org/10.1039/d3ra04555b
• Petpiroon N, Netkueakul W, Sukrak K, Wang C, Liang Y, Wang M, Liu Y, Li Q, Kamran R, Naruse K, Aueviriyavit S, Takahashi K. Development of lung tissue models and their applications. Life Sci. 2023 Dec 1;334:122208. https://doi.org/10.1016/j.lfs.2023.122208
• Wongwanakul R, Aueviriyavit S, Furihata T, Gonil P, Sajomsang W, Maniratanachote R, Jianmongkol S. Quaternization of high molecular weight chitosan for increasing intestinal drug absorption using Caco-2 cells as an in vitro intestinal model. Sci Rep. 2023 May 16;13(1):7904. https://doi.org/10.1038/s41598-023-34888-0
• Netkueakul W, Chortarea S, Kulthong K, Li H, Qiu G, Jovic M, Gaan S, Hannig Y, Buerki-Thurnherr T, Wick P, Wang J. Airborne emissions from combustion of graphene nanoplatelet/epoxy composites and their cytotoxicity on lung cells via air-liquid interface cell exposure in vitro. NanoImpact. 2022 Jul;27:100414. https://doi.org/10.1016/j.impact.2022.100414
• Kitipaspallop W, Sillapaprayoon S, Phuwapraisirisan P, Kim WK, Chanchao C, Pimtong W. Developmental effects of sesamolin on zebrafish (Danio rerio) embryos. Comp Biochem Physiol C Toxicol Pharmacol. 2022 Jun;256:109319. https://doi.org/10.1016/j.cbpc.2022.109319
• Petpiroon N, Rosena A, Pimtong W, Charoenlappanit S, Koobkokkruad T, Roytrakul S, Aueviriyavit S. Protective effects of Thai silk sericins and their related mechanisms on UVA-induced phototoxicity and melanogenesis: Investigation in primary melanocyte cells using a proteomic approach. Int J Biol Macromol. 2022 Mar 15;201:75-84. https://doi.org/10.1016/j.ijbiomac.2021.12.061
• Klinngam W, Rungkamoltip P, Thongin S, Joothamongkhon J, Khumkhrong P, Khongkow M, Namdee K, Tepaamorndech S, Chaikul P, Kanlayavattanakul M, Lourith N, Piboonprai K, Ruktanonchai U, Asawapirom U, Iempridee T. Polymethoxyflavones from Kaempferia parviflora ameliorate skin aging in primary human dermal fibroblasts and ex vivo human skin. Biomed Pharmacother. 2022 Jan;145:112461. https://doi.org/10.1016/j.biopha.2021.112461